Susan Gordehie; Reza Sheikhnejad; Seyed Abdolhamid Angaji; Mahmoud Khanaki; Vahid Marandi
Volume 25, Issue 1 , May and June 2018, , Pages 60-68
Abstract
Background and Purpose: Pancreatic cancer is one of the most leading causes of cancer-related deaths. Considering the role of Bcl-2 family gene in apoptosis that are known to be over-expressed in most cancers, this study focused on the detection of Bcl-2 translocation t(14;18) to the immunoglobulin heavy ...
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Background and Purpose: Pancreatic cancer is one of the most leading causes of cancer-related deaths. Considering the role of Bcl-2 family gene in apoptosis that are known to be over-expressed in most cancers, this study focused on the detection of Bcl-2 translocation t(14;18) to the immunoglobulin heavy chain (IgH) that may contribute to the pathogenesis of pancreatic cancer. Materials and Methods: Forty-nine samples of paraffin embedded tissues (extracted from 1537 slides of 105 patients in one of the major local cancer centers) were investigated for detection of Bcl-2 translocation t(14;18) by standard PCR. Results: The Bcl-2 t(14;18) translocation was detected in 23 of all 49 patients (46.9%), including 10 of 23 translocated patients (43%) with break points within the mbr cluster, 11 with involvement of the mcr locus (48%), and 2 in the icr locus (9%). Discussion: Detection of Bcl2 translocation in pancreatic cancer is in agreement with results from other studies, and shows this rearrangement could be considered as a new treatment strategy based on apoptosis or personalized treatment. Keywords: apoptosis, Bcl-2 translocation, pancreatic cancer,paraffin embedded tissue, personalized treatment, polymerase chain reaction.